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human fetal liver cdna expression library  (TaKaRa)


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    Structured Review

    TaKaRa human fetal liver cdna expression library
    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
    Human Fetal Liver Cdna Expression Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fetal+liver+cdna+library/Human+Liver+QUICK-Clone+cDNA/pmc01257442-107-21-20
    Average 94 stars, based on 597 article reviews
    human fetal liver cdna expression library - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Identification of kinectin as a novel Behçet's disease autoantigen"

    Article Title: Identification of kinectin as a novel Behçet's disease autoantigen

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar1798

    cDNA clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of the candidate clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
    Figure Legend Snippet: cDNA clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of the candidate clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.

    Techniques Used: Clone Assay, Expressing, Sequencing, Derivative Assay, Autoradiography, In Vitro, Labeling, SDS Page, Positive Control

    Related Articles

    Amplification:

    Article Title: Blood coagulation factor VII and VIIa derivatives, conjugates and complexes comprising the same, and use thereof
    Article Snippet: .. For amplification of Factor VII (FacVII) gene, a human fetal liver cDNA library (TAKARA BIO USA) was used as a template, and forward and reverse primers of the following SEQ ID NOs. ..

    Article Title: Medium containing uridine and N-acetyl-D-mannosamine
    Article Snippet: .. [Construction of Expression Vector for Human Erythropoietin] cDNA encoding human erythropoietin was PCR amplified from human fetal liver cDNA library (Clontech Inc.) using the following set of primers: Primer EPO-5′, synthetic sequence (SEQ ID NO:1) and Primer EPO-3′, synthetic sequence (SEQ ID NO:2). ..

    cDNA Library Assay:

    Article Title: Blood coagulation factor VII and VIIa derivatives, conjugates and complexes comprising the same, and use thereof
    Article Snippet: .. For amplification of Factor VII (FacVII) gene, a human fetal liver cDNA library (TAKARA BIO USA) was used as a template, and forward and reverse primers of the following SEQ ID NOs. ..

    Article Title: Sequences encoding
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: Medium containing uridine and N-acetyl-D-mannosamine
    Article Snippet: .. [Construction of Expression Vector for Human Erythropoietin] cDNA encoding human erythropoietin was PCR amplified from human fetal liver cDNA library (Clontech Inc.) using the following set of primers: Primer EPO-5′, synthetic sequence (SEQ ID NO:1) and Primer EPO-3′, synthetic sequence (SEQ ID NO:2). ..

    Article Title: Polypeptides and nucleic acids encoding same
    Article Snippet: .. To isolate the full-length human clone corresponding to 65.11.3 (mouse clone 65), a human fetal liver cDNA library (Clontech), treated with the SuperScriptTM kit using the pRK5E vector as described above, was screened with a probe (65.50.mer.2 (SEQ ID NO:42) noted above in Example 1) at low stringency (20% formamide, 1×SSC, 55° C. wash). ..

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Article Title:
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: ADAMTS13 genes and proteins and variants and therapeutic compositions and methods utilizing the same
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products.

    Article Title: Desmoplakin and KIF20B as target antigens in patients with paroxysmal nocturnal haemoglobinuria.
    Article Snippet: .. We screened a human fetal liver cDNA library (Takara Bio Europe, SaintGermain-en-Laye, France) known to contain a high proportion of cells derived from haematopoietic stem/progenitor cell (HSPC). .. Briefly, XL1-Blue Escherichia coli (Takara Bio Europe, Saint-Germain-en-Laye, France) was transfected with recombinant phages, plated on agar plates, and cultured at 37 C. Expression of recombinant proteins was induced by incubating the bacterial lawns with isopropyl b-D-thiogalactoside (IPTG).

    Derivative Assay:

    Article Title: Sequences encoding
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title:
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: ADAMTS13 genes and proteins and variants and therapeutic compositions and methods utilizing the same
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products.

    Article Title: Desmoplakin and KIF20B as target antigens in patients with paroxysmal nocturnal haemoglobinuria.
    Article Snippet: .. We screened a human fetal liver cDNA library (Takara Bio Europe, SaintGermain-en-Laye, France) known to contain a high proportion of cells derived from haematopoietic stem/progenitor cell (HSPC). .. Briefly, XL1-Blue Escherichia coli (Takara Bio Europe, Saint-Germain-en-Laye, France) was transfected with recombinant phages, plated on agar plates, and cultured at 37 C. Expression of recombinant proteins was induced by incubating the bacterial lawns with isopropyl b-D-thiogalactoside (IPTG).

    Clone Assay:

    Article Title: Sequences encoding
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title:
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: ADAMTS13 genes and proteins and variants and therapeutic compositions and methods utilizing the same
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products.

    Isolation:

    Article Title: Sequences encoding
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title:
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: ADAMTS13 genes and proteins and variants and therapeutic compositions and methods utilizing the same
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Sequences encoding
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title:
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. An error in the 3′ RACE clone (insertion of a G at position 3631 of AF414401) was corrected by site-directed mutagenesis using the GeneEditor mutagenesis system (Promega).

    Article Title: ADAMTS13 genes and proteins and variants and therapeutic compositions and methods utilizing the same
    Article Snippet: .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products. .. Nucleotides 1-707 (with the A of the ATG designated +1) were derived from IMAGE EST clone 1874472 (GenBank accession number AI281246); nucleotides 708-896, and 897-1748 were derived from two previously described cDNA clones isolated from a human fetal liver cDNA library (Clontech), nucleotides 1749-2918 and 2919-4329 were derived from previously described RT-PCR and 3′ RACE products.

    Expressing:

    Article Title: Medium containing uridine and N-acetyl-D-mannosamine
    Article Snippet: .. [Construction of Expression Vector for Human Erythropoietin] cDNA encoding human erythropoietin was PCR amplified from human fetal liver cDNA library (Clontech Inc.) using the following set of primers: Primer EPO-5′, synthetic sequence (SEQ ID NO:1) and Primer EPO-3′, synthetic sequence (SEQ ID NO:2). ..

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Polymerase Chain Reaction:

    Article Title: Medium containing uridine and N-acetyl-D-mannosamine
    Article Snippet: .. [Construction of Expression Vector for Human Erythropoietin] cDNA encoding human erythropoietin was PCR amplified from human fetal liver cDNA library (Clontech Inc.) using the following set of primers: Primer EPO-5′, synthetic sequence (SEQ ID NO:1) and Primer EPO-3′, synthetic sequence (SEQ ID NO:2). ..

    Sequencing:

    Article Title: Medium containing uridine and N-acetyl-D-mannosamine
    Article Snippet: .. [Construction of Expression Vector for Human Erythropoietin] cDNA encoding human erythropoietin was PCR amplified from human fetal liver cDNA library (Clontech Inc.) using the following set of primers: Primer EPO-5′, synthetic sequence (SEQ ID NO:1) and Primer EPO-3′, synthetic sequence (SEQ ID NO:2). ..

    Transfection:

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Negative Control:

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Construct:

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Selection:

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).

    Western Blot:

    Article Title: Expression of phosphoenolpyruvate carboxykinase linked to chemoradiation susceptibility of human colon cancer cells.
    Article Snippet: The membranes were washed, incubated with diluted HRP-conjugated secondary antibody (SouthernBiotech, Birmingham, AL), and exposed to film (Blue XB-1; Kodak, Rochester, NY). .. The level of intracellular PEP was measured using a PEP assay kit (BioVision Inc., Milpitas, CA) as recommended by the manufacturer. siRNA transfection Transfection of mPEPCK siRNA (Santa Cruz Biotechnology, Santa Cruz, CA) and negative control siRNA (Qiagen, Chatsworth, CA) was performed with the HiPerFect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. mPEPCK expression construct To generate pEGFPc1-mPEPCK, a human fetal liver cDNA library (Clontech, Mountain View, CA) was PCRamplified with the following oligomers specific to human Figure 1 Downregulated mitochondrial phosphoenolpyruvate carbox with induced 5-FU resistance. (a) Selection of downregulated protein sp protein was identified as being downregulated in SNU-C4R. (b) Identificatio identified as mPEPCK. (c) Western Blot analysis for confirmation of downreg isoforms) and mPEPCK expression was significantly lower in SNU-C4R than in mPEPCK (Macrogen, Seoul, Korea): sense, 5′-GGAATT CCATGGCCGCATTGTACCGCC-3′ and antisense, 5′- CGGGATCCTCAGGTCACATTTTGTGCACACGTC-3′. .. The amplified DNA was digested with EcoRI-BamHI and then inserted into pEGFPc1 (Clontech).



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    human fetal liver cdna expression library  (TaKaRa)
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    TaKaRa human fetal liver cdna expression library
    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
    Human Fetal Liver Cdna Expression Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fetal+liver+cdna+library/Human+Liver+QUICK-Clone+cDNA/pmc01257442-107-21-20
    Average 94 stars, based on 1 article reviews
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    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
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    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
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    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
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    <t>cDNA</t> clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of <t>the</t> <t>candidate</t> clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.
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    Image Search Results


    cDNA clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of the candidate clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.

    Journal: Arthritis Research & Therapy

    Article Title: Identification of kinectin as a novel Behçet's disease autoantigen

    doi: 10.1186/ar1798

    Figure Lengend Snippet: cDNA clones obtained from expression library screen using a BD serum. (a) Schematic diagram of the six overlapping cDNAs aligned with published human full-length kinectin sequence (4,816 bp, GenBank accession number NM_182926 , shown on top). The open box represents the coding region of the full-length kinectin protein of ~156 kDa. cDNA inserts from the six independent clones BD41/BD44, BD481, BD42, BD47, BD482 and BD49 represent N-terminal truncations and are predicted as coiled-coil domains (hatched). ▽ represents short sequences derived from alternative mRNA splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of the candidate clones BD44 and BD42 labeled by [ 35 S]-methionine and analyzed on a 12.5% gel SDS-PAGE. Products of BD44 and BD42 gave major bands with the highest molecular mass of 95 kDa and 60 kDa, respectively. The lane marked as p90 represents an unrelated autoantigen used as a positive control for the in vitro translation reaction. Molecular markers are shown on the left. BD, Behçet's disease.

    Article Snippet: The initial report by Hirano et al. identified kinectin by screening an aplastic anemia patient for candidate antigens using a Clontech human fetal liver cDNA expression library and it was concluded that seven out of 18 aplastic anemia patients were positive for anti-kinectin while none of the normal or disease controls had this antibody [ ].

    Techniques: Clone Assay, Expressing, Sequencing, Derivative Assay, Autoradiography, In Vitro, Labeling, SDS Page, Positive Control